LIST OF RESEARCH PUBLICATION

Dhungel, B., Subedi, M., Tiwari, K.B., Shrestha, U.T., Pokhrel, S. and Agrawal, V.P. (2007) Characterization of glucose isomerase from cold tolerant Streptomyces species. (Completed article).

Shah, S.K., Tiwari, K.B., Shrestha, U.T., Pokhrel, S., Agrawal, V.K. and Agrawal V.P. (2007). Cloning of cry3 fragment in Escherichia coli. (Completed article)

Shrestha, U.T., Tiwari, K.B. and Agrawal, V.P. (2007). Strong mosquitocidal Bacillus thuringiensis from Mt. Everest. (Completed report).

Sahukhal, G.S., Shrestha, U. T., Tiwari, K.B., Lekhak, B., Singh, A. and Agrawal, V.P. (2007) Genomic fingerprinting of Bacillus thuringiensis isolates from Khumbu, Everest base camp by RAPD-PCR. Journal of Nepal Biotechnology Association (accepted).

Sahukhal, G.S., Shrestha, U. T., Tiwari, K.B., Lekhak, B., Singh, A. and Agrawal, V.P. (2007) Optimization of RAPD-PCR for genetic polymorphisms of Bacillus thuringiensis. Submitted to Journal of Institute of Science and Technology (Nepal).

**Bhattarai K, Tiwari KB and Agrawal VP (2007). LOF and GOF mutations in Streptomyces spp. by sodium azide. Journal of Nepal Biotechnology Association (accepted report).

**Shrestha ML, Tiwari KB and Agrawal VP (2007). Abundance of lactose assimilating yeasts from Nepalese Murcha (Yeast Cake). Journal of Nepal Biotechnology Association (accepted).

**Bhattarai K, Tiwari KB and Agrawal VP (2007). Enhanced antibacterial activity of sodium azide treated mutant Streptomyces strain. Journal of Nepal Association for Medical Laboratory Sciences , 8(1): 67-8.

*Shrestha UT, Sahukhal GS, Pokharel S, Tiwari KB, Singh A, Agrawal VP(2006) Delta Endotoxin Immunocrossreactivity of Bacillus thuringiensis isolates collected from Khumbu Base Camp of Mount Everest Region. J. Food Sci. & Technol. Nepal 2:128-131

** Singh D and Agrawal VP (2002) Microbial Biodiversity of Mount Sagarmatha Region, a paper presented in the International Seminar on Mountains, Kathmandu March 6 - 8, 2002.

*Agrawal VP (2001) Fatty Acid Isopropylidene Hydrazides - A Novel Class of Derivatives for Analysis of Fatty Acids. Nepal J. Science Technology 123-131.

*Singh D and Agrawal VP (2001) Trouble - Shooting in Sodium Dodecyl Sulphate Poly-acryalamide Gel Electrophoresis. In Microbes Newsletter Vol 3(2) published by Nepalese Society for Microbiology.

*Rakwal R, Agrawal GK, Tamogami S, Yonekura M, Agrawal VP, Shibato J and Iwahashi H (2004) Differential activation of phytoalexin production and induction of proteins by amino acid conjugates of jasmonic acid in rice (Oryza sativa L.) seedling leaves . Nepal J. Sci. & Tech. 00: 000-000.

*Rakwal R, Agrawal GK, Tamogami S, Yonekura M, Agrawal VP and Iwahashi H (2003) Novel insight into kinetin-inducible stress responses in rice seedlings. Plant Physiol. Biochem . 41: 453-457.

*Agrawal GK, Rakwal R and Agrawal VP (2003) Regulation of OsRac1 mRNA against defense-related stimuli in wild-type rice seedlings . Biol. Plant . 46: 551-556.

*Agrawal GK, Jwa NS, Han KS, Agrawal VP and Rakwal R (2003) Isolation of a novel rice PR4 type gene whose mRNA expression is modulated by blast pathogen attack and signaling components. Plant Physiol. Biochem . 41: 81-90.

*Agrawal GK, Tamogami S, Iwahashi H, Agrawal VP and Rakwal R (2003) Transient regulation of jasmonic acid-inducible rice MAP kinase gene (OsBWMK1 ) by diverse biotic and abiotic stresses. Plant Physiol. Biochem. 41: 355-361.

*Jwa NS, Agrawal GK, Rakwal R, Kim JA and Agrawal VP (2002) Differential regulation of an early and late responsive rice endosperm kinase mRNA in seedling leaves . Plant Physiol. Biochem. 40: 1025-1031.

*Agrawal GK, Rakwal R, Jwa NS, Han KS and Agrawal VP (2002) Molecular cloning and mRNA expression analysis of the first rice jasmonate biosynthetic pathway gene allene oxide synthase . Plant Physiol. Biochem. 40: 771-782.

*Agrawal GK, Rakwal R, Jwa NS and Agrawal VP (2002) Characterization of a novel rice gene OsATX and modulation of its expression by components of the stress signalling pathways . Physiol. Plant. 116: 87-95.

*Agrawal GK, Rakwal R, Jwa NS and Agrawal VP (2002) Oryza sativa proteinase inhibitor gene, OsPIN , whose mRNA specifically accumulates in a compatible host-blast pathogen interaction. Plant Physiol. Biochem. 40: 175-182.

*Agrawal GK, Rakwal R, Jwa NS and Agrawal VP (2002) Effects of signaling molecules, protein phosphatase inhibitors and blast pathogen ( Magnaporthe grisea ) on the mRNA level of a rice ( Oryza sativa L.) phospholipid hydroperoxide glutathione peroxidase ( OsPHGPX ) gene in seedling leaves . Gene 283: 227-236.

*Agrawal GK, Rakwal R, Jwa NS and Agrawal VP (2001) Signaling molecules and blast pathogen attack activates rice OsPR1a and OsPR1b genes: A model illustrating components participating during defense/stress response. Plant Physiol. Biochem. 39: 1095-1103.

*Rakwal R, Agrawal GK, and Agrawal VP (2001) Jasmonate, salicylate, protein phosphatase 2A inhibitors and kinetin up-regulate OsPR5 expression in cut-responsive rice ( Oryza sativa ). J. Plant Physiol. 158: 1357-1362.

*Jwa NS, Agrawal GK, Rakwal R, Park CH and Agrawal VP (2001) Molecular cloning and characterization of a novel jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10 , from rice ( Oryza sativa L.) seedling leaves. Biochem. Biophys. Res. Commun. 286: 973-983.

*Nirmal Joshee, D.R. Bastola, V.P. Agrawal and A.K. Yadav (2002) Lakoocha: A Multipurpose Tree of Warm Climate Trends in new crops and new uses. 2002. J. Janick and A. Whipkey (eds.). ASHS Press, Alexandria, VA.

*Dhundy R. Bastola, V. P. Agrawal and Nirmal Joshee (2000) In vitro propagation of a Himalayan pine P. wallichiana A. B. Jacks Current Science, vol. 78, No. 3, 338-341

*B. N. Prasad (Editor), G. P. S. Ghimire (Editor) and V. P. Agrawal (Editor) Role of Biotechnology in Agriculture Publisher : Science Publishers, Inc.; Reprint edition (August 1995) ISBN: 1881570126

*Keshari AK, Singh RL, Agrawal SK, Agrawal GK and Agrawal VP (1994) Rice anther culture in RLABB. Rice Biotechnol. Q. 19: 8.

*Agrawal, S.K., Keshari, A.K., Singh, R.L., Agrawal GK and Agrawal, V.P., (1994) Plant regeneration from anthers of Oryza sativa L. cv. Jumli Marshi and Chhomrong-two Cold tolerant Rice Varieties of Nepal, Plant Tissue Cult. Vol. 4(1), pp. 25-31.

*Keshari, A.K., Singh, S.K., Agrawal, S.K., Agrawal GK and Agrawal, V.P., (1994) Anther culture of rice cultivars of Nepal, Rice Biotech. Quart. Vol. 20, pp.7.

*Agrawal GK and Agrawal, V.P., (1994) Variation in degree of cold tolerance of anther derived rice seeds, Rice Biotech. Quart. Vol. 20, p0p. 23.

*Gautam, S.K. and Agrawal, V.P., (1994) Measurement of heavy metals in Bagmati river and its tributaries flowing through Kathmandu Valley, a M.Sc. Thesis submitted to Tribhuvan University, Kathmandu, Nepal

*Sigdel, T.K. and Agrawal, V.P., (1994) Study of Zinc deficiency problem in paddy fields of Eastern Pokhara, a M.Sc. Thesis submitted to Tribhuvan University, Kathmandu, Nepal.

*Shakya NM, Mulmi DD, Wagley DP and Agrawal VP (1994) The calculation of Ground State Hydrogen Molecule, Ion and Helium Atom by Single Centre Expansion Method using Newly Constructed Wave Function called Gaussian Shell Orbital. Proceedings of 2nd National Conference on Science and Technology June 8-14, pp. 418 - 424.

*Agrawal GK and Agrawal, V.P., (1993) Esterase isozyme bands used as a marker in rice, Rice Biotech. Quart. Vol. 12, pp. 11.

*Shakya, S., Agrawal GK and Agrawal, V.P., (1993) An improved ribulose bis phosphate carboxylase method for differentiating rice lines, Rice Biotech. Quart. Vol. 14, pp. 25.

*Shakya, S. And Agrawal, V.P., (1993) Rubisco screening for cold tolerance in rice, Plant Physiol. (Supplement) Vol. 102, pp. 442.

*Shamim, A.S. and Agrawal, V.P. (1993) Differential inhibition of cold tolerant and sensitive rice cultivars by short chain saturated fatty acids, Biotechnol. Biodivers. Lett. Vol. 3, pp. 1-7.

*Singh, M.K., Agrawal GK and Agrawal V.P. (1993) Effect of chemical mutagens on seed germination of Brassica campestris var toria, Biotechnol. Biodivers. Lett. Vol. 3, pp. 14-17.

*Prasad, B.N., Ghimire, G.P.S. and Agrawal, V.P., (1992) Role of biotechnology in agriculture, Oxford and IBH Publishing Co. Pvt. Ltd.

*Keshari, A.K., Singh, R.L., Agrawal, S.K., Agrawal GK and Agrawal, V.P., (1992) Studies on anther culture of rice cultivars of Nepal, Biotech. Biodiv. Lett. Vol. 2, pp. 1-10.

*Agrawal GK, Khandka, D.K., Chaudhary, R.P. and Agrawal, V.P., (1992) Screening of Maleness related protein in Choerospondias axillaris seedlings and a sex marker, Biotech. Biodiv. Lett. Vol. 2, pp. 11-15.

*Shrestha, J.N. and Agrawal, V.P., (1992) Improvement of inverted embryo technique for pine micropropagation – role of light, Biotech. Biodiv. Lett. Vol. 2, pp. 16-18.

*Agrawal GK, Pandy, R.N. and Agrawal, V.P., (1992) Isolation of DNA from Choerospondias axillaris Leaves, Biotech. Biodiv. Lett. Vol. 2, pp. 19-24.

*Baral, J.B., Singh, R.L., Chaudhary, R.P. and Agrawal, V.P., (1992) Collection of indigenous rice germplasm, Biotech. Biodiv. Lett. Vol. 2, pp. 25-26.

*Shakya, S., Joshee, N. and Agrawal, V.P., (1992) Commercial potential of inverted embryo technique for in vitro propagation of Pinus wallichiana, Ban Ko Jankari, Vol. 3(3), pp. 29-32.

*Agrawal, S.K., Keshari, A.K., Singh, R.L., Shrestha, K.P. and Agrawal, V.P., (1991) Comparative Study on Anther Culture of Jumli Marshi and Chhomrong -Two important cold tolerant rice cultivars of Nepal, Biotech. Biodiv. Lett. Vol. 1, pp. 1-4.

*Agrawal GK and Agrawal V.P., (1991) Biochemical study of albinism in rice, a thesis submitted to Tribhuvan University, Kathmandu, Nepal.

*Agrawal GK, Thapa, A., Keshari, A.K., Manandhar, S.P. and Agrawal, V.P., (1991) Restriction fragment length polymorphism technique for rice research Biotech. Biodiv. Lett. Vol. 1, pp. 5-9.

*Agrawal GK and Agrawal, V.P., (1991) Study on haploid and diploid plants of cold tolerant rice cultivar Jumli Marshi, Biotech. Biodiv. Lett. Vol. 1, pp. 10-11.

*Joshee, N., Malla, L. and Agrawal, V.P., (1991) Comparative potential of callus induction and plant regeneration from seeds of cold tolerant and sensitive rice cultivars grown in Nepal, Biotech. Biodiv. Lett. Vol. 1, pp. 12.

*Shamim, S. A. and Agrawal, V.P., (1991) Biochemistry of saturated short chain fatty acids induced inhibition of rice seed germination, Biotech. Biodiv. Lett. Vol. 1, pp. 13.

*Agrawal, V.P., Shakya, S., Bastola, D.R. and Joshee, N., (1991) Inverted embryo technique for in vitro Propagation of Himalayan Pines P. roxburghii and P. wallichiana, Biotech. Biodiv. Lett. Vol. 1, pp. 14-19.

*Agrawal, S.K. and Agrawal, V.P., (1991) Relation between production of phenolic compounds and the age of embryo during callus induction of p. wallichiana, Biotech. Biodiv. Lett. Vol. 1, pp. 20.

*Joshee, N., Bastola, D.R., Yadav, B.K. and Agrawal, V.P., (1991) Clonal propagation of Artocarpus lakoocha (Badahar), Biotech. Biodiv. Lett. Vol. 1, pp. 21.

*Agrawal, S.K. and Agrawal, V.P., (1991) Callus induction in Brassica campestris var toria, Biotech. Biodiv. Lett. Vol. 1, pp. 22.

*Agrawal GK, Khandka, D.K., Jha, B., Thapa, A., Tamrakar, S., Manandhar, S.P., Chaudhary, R.P. and Agrawal, V.P., (1991) Studies on methods for early sex determination of Choerospondias axillaris, Biotech. Biodiv. Lett. Vol. 1, pp. 23-28.

*Khandka, D.K., Agrawal GK and Agrawal, V.P., (1991) Effect of sodium dodecyl sulphate on protein-dye complex of bovine serum albumin (BSA) and coomassie brilliant blue, Biotech. Biodiv. Lett. Vol. 1, pp. 29-30.

*Agrawal GK and Agrawal, V.P., (1991) Effect of light on biochemical reactions in plants, Biotech. Biodiv. Lett. Vol. 1, pp. 31-32.

*Manandhar, S.P., Thapa, A. and Agrawal, V.P., (1991) Screening of the medicinal properties of some plants of Nepal, Biotech. Biodiv. Lett. Vol. 1, pp. 33-36.

*Shamim, S.A. and Agrawal, V.P., (1991) Biochemical study on lipids of pinus roxburghii needles, Biotech. Biodiv. Lett. Vol. 1, pp. 37.

*Khatri, G.B., Jha, P.K., Agrawal, V.P. and Jacobsohn, R., (1991)Distribution, host range and phenology of Orobanche spp. In Nepal, in Progress in Orobanche Research, pp. 18-24.

*Jacobsohn, R., Kleifeld, Y., Agrawal, V.P., Jha, P.K. and Marton, K., (1991) Soil fumigation with Telon II for broomrape (Orobanche spp.) control, in Progress in Orobanche Research, pp. 185-190.

*Joshee, N., Bastola, D.R. and Agrawal, V.P. (1989) Commercial potential of inverted embryo technique for in vitro propagation of Pinus roxburghii. Proceedings of the International Symposium on Application of Biotechnological and Recent Accomplishments of Economic Value in Asia, No. 5-9, 1989, Bangkok.

*Rajbhandari, M.L. and Agrawal, V.P. (1987) A modified method for determining phosphorous in phopholipids, J. Nepal Chem. Soc. Vol. 5-7, pp. 6-11.

*Rajbhandari, M.L. and Agrawal, V.P. (1987) Proton magnetic resonance spectra of some fatty acid isopropylidene hydrazides, J. Nepal Chem. Soc. Vol. 5-7, pp. 29-37.

*Agrawal, V.P. Regulation of biosynthesis of saturated and unsaturated long chain fatty acids in Plants . J. Institute of Science, T.U. in Press.

*Shrestha, P.P. and Agrawal, V.P. Phytochemical studies on cyanogenic plant of Nepal : Screening of cyanogenic plants of rosaceae family, submitted for publication as proceeding on the National S/T conference organised by RONAST. April 1988.

*Agrawal, V.P. and Stumpf, P.K. (1985) Characterization and solubilization of a acyl chain elongation system in microsomes of leek epidermal cells, Archives Biochem. Biophys.240: 154-165.

*Agrawal, V.P. and Stumpf, P.K. (1985) Elongation systems involved in the biosynthesis of erucic acid from oleic acid in developing Brassica Juncea seeds. Lipids 20: 361-366.

*Sanchez, J., Agrawal, V.P. and Stumpf, P.K. (1984) Metabolism of C-14-Oleoyl-CoA by safflower microsomes; The effect of ATP and CoA, in "Structure, function and metabolism of Plant lipids" (eds. P.A. Siegenthaler and W. Eichenberger). Pp 70-80, Elsevier Science publishers.

*Lessire, R., Agrawal, V.P. and Stumpf, P.K. (1984) Fatty acid biosynthesis in Allium porrum L. Leaves. In eds. P.A. Siegenthaler and W. Eichenberger "Structure, function and metabolism of plant lipids". Elsevier Science Publishers. Pp. 11-15.

*Agrawal, V.P., Lessire, R. and Stumpf, P.K. (1984) Biosynthesis of very long chain fatty acids in microsome from epidermal cells of Allium porrum L. Archives Biochem. Biophys 230:580-589.

*Agrawal, V.P. (1984) Mass Spectra of N'-Isopropylidenealkanohydrazides, Org. Mass Spectrom 19: 196.

*Agrawal, V.P. and Kolattukudy, P.E. (1984) Mass spectral studies of 2-Alkyl-4, 4-dimethyl-2-oxazolines, Org. Mass Spectrom 19: 249-250.

*Agrawal, V.P., and Blagrove, R.J., (1984) A novel method for spectrophotometric determination of triglycerides, J. Amer. Oil Chemists' Soc. 61: 766-768.

*Agrawal V.P. (1983) Gas-liquid chromatography and high performance liquid chromatography of cyclopentenyl fatty acid isopropylidene hydrazides and their identification by mass spectrometry, J. Nepal Chem. Soc. 3: 1-10.

*Agrawal, V.P. (1983) High performance liquid chromatography of fatty acid isopropylidene hydrazides and its application in lipid analysis, Anal. Biochem. 131: 356-359.

*Blagrove, R.J. and Agrawal, V.P., (1982) Lupin seed as a source of oil and protein. In: eds. J.N. Hawthrone and D. Lekin "Soya Lecithin Dietect Application" pp. 55-71.

*Agrawal, V.P. (1983) Hydrazinolysis of lipids and analysis of constituent fatty acids. J. Lipid Research 24: 216-220.

*Agrawal, V.P. (1983) Hydrazinolysis of lipids and analysis of constituent fatty acids. J. Lipid Research 24: 216-220.

*Kolattukudy, P.E. and Agrawal, V.P. (1981) ?-Hydroxy Fatty acid: NADP oxidoreductase from wound healing potato tuber disks. Methods in Enzymology 71: 411-420.

*Agrawal, V.P. and Poulose A.J. (1981) Comparative study of mass spectra of trimethylsilyl ester derivatives of cis-and trans-3, 7-dimethylocta-2, 6-dienoic acids. J. Nepal chem. Soc. 1: 1-8.

*Agrawal, V.P. (1978) Biochemistry of a wound – induced ? - hydroxy fatty Acid: NADP oxidoreductase from Potato tuber a key enzyme involved in suberization, Ph.D. Dissertation, Washington State University, Pullman.

*Agrawal, V.P. and Kolattukudy, P.E. (1978) Mechanism of action of a wound-induced ?-hydroxyfatty acid, NADP oxidoreductase from potato tuber Solanum tuberosum L. Archives Biochem. Biophys. 191: 466-478.

*Agrawal, V.P. and Kolattukudy, P.E. (1978) Purification and characterization of a wound-induced W-hydroxy fatty acid: NADP: oxidoreductase from potato tuber disks (Solanum tuberosum L.). Archives Biochem. Biophys. 191: 452-465.

*Agrawal, V.P. (1977) Study on the suitability of gaussian shell orbitals (GSO) as a basis Set for single-center expansion method. M.S. Thesis Washington State University, Pullman.

*Agrawal, V.P. and Kolattukudy, P.E. (1977) Biochemistry of suberization: ?-hydroxyacid oxidation in enzyme preparations from suberizing potato tuber disks. Plant Physiol 59: 667-672.

*Agrawal, V.P. and Kolattukudy, P.E. (1974) Structure and composition of aliphatic constituents of potato tuber skin (suberin). Lipids 9: 682-691.

*Poshusta, R.D., Agrawal, V.P. and Moseley, W.D. (1975) On the use of Gaussian type basis orbitals for single-center expansion. I. Evaluation of Integrals. International J. of Quantum Chem. 9: 682-691.

*Poshusta, R.D. and Agrawal, V.P. (1973) Stability of HeH3. J. Chem. Phys. 59:2744-2482.

*Amatya, D.M.S. and Agrawal, V.P. (1973) Kinetic studies on the reaction between chromium (III) and thiocyanate ions in methanol – water at different pH values. J. Tribhuvan University *: 72-80.

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